郭良宏

发布者:admin2发布时间:2016-01-15浏览次数:546


姓名:郭良宏性别:男                     
职称:研究员  电话:62849685
Email:LHGuo@rcees.ac.cn邮编:100085
简历:
    郭良宏,研究员,博士生导师,国家基金委杰出青年基金获得者,中国科学院百人计划入选者,国家科技部重大科学研究计划首席科学家。1985年本科毕业于中国科学技术大学应用化学系,1985-1987在武汉大学化学系做硕士研究生,1991年获得英国牛津大学无机化学系博士学位, 1991-1995年在美国Rochester大学化学系进行博士后研究,2004加入中国科学院生态环境研究中心。
研究方向:
主要从事持久性有机污染物的分子毒理和生物检测方法研究。
 
社会任职:

环境化学与生态毒理学国家重点实验室副主任

环境健康研究中心副主任

中国环境科学学会环境医学与健康分会委员

中国毒理学会分析毒理专业委员会委员

中国仪器仪表学会分析仪器分会电化学分析专业委员会委员

北京色谱学会副理事长

中国化学会质谱分析专业委员会委员

英国皇家化学会RSC期刊Environmental Science: Processes and Impacts副主编

《环境化学》常务副主编

《科学通报》编委

《分析化学》编委

《生态毒理学报》编委


承担科研项目:

1.   国家自然科学基金委面上项目:“DNA损伤的电化学发光传感检测方法与应用研究”2014.01-2017.12

2.   国家科技部重大科学研究计划:“纳米材料的水处理器件化方法及其应用基础研究”2010.10-2015.08

3.   国家自然科学基金委面上项目:“基于酪氨酸电化学的蛋白质磷酸化检测”  2009.01-2011.12

4.   国家自然科学基金委杰出青年基金项目:“生物分析化学”  2009.01-2012.12

5.   国家自然科学基金委重大项目课题:“典型持久性污染物的累积代谢及其与生物分子的相互作用”2009.01-2012.12

6.   中国科学院重大科研装备研制项目:“环境化合物遗传毒性快速检测仪”  2008.03-2010.06  

7.   国家自然科学基金委创新研究群体:“持久性有毒污染物形态、环境过程与毒理效应(参与)”  2007.01-2010.12

8.   国家科技部高技术研究发展计划(863)项目:“环境中具有遗传毒性化学污染物的光电化学快速筛查技术”  2007.01-2009.12

9.   国家自然科学基金委面上项目:“电化学方法研究环境化合物与核酸的相互作用及其基因毒理”  2007.01-2009.12

10.  中国科学院“百人计划”项目:“生物分析与生态毒理”  2004.06-2007.05 

获奖及荣誉:

2004    中国科学院“百人计划”

2009    国家自然基金委杰出青年基金

2009    中国科学院优秀研究生指导教师

 

代表论著:

1.    Qiang Ma*, Hua Bai, Chao Wang, Guang-Cheng Xi, Qing Zhang, Xian-Shuang Meng, Yun-Xia Chen, Jing-Rui Li, Hui-Juan Ma, Liang-Hong Guo*, “Investigation of noncovalent interactions between hydroxylatedpolybrominated diphenyl ethers and bovine serum albumin usingelectrospray ionization-ion mobility-mass spectrometry”, International Journal of Mass Spectrometry, 2014, 357, 34-44.

2.    刘世利, 陈守臻, 赵倩, 徐政虎,李玉贾,继辉, 郭良宏*, “光电化学竞争法检测生物素”, 《分析化学》, 2013, 10, 1477 -1481.

3.    Lianying Zhang, Xiao-Min Ren, and Liang-Hong Guo*, “Structure-Based Investigation on the Interaction of Perfluorinated Compounds with Human Liver Fatty Acid Binding Protein”, Environ. Sci. Technol. 2013, 47, 11293−11301.

4.    李敏杰, 吴一萍, 张斌田, 郭良宏* , “DNA 脱碱基位点的检测与应用”, 《分析化学》,2013, 7, 965-972.

5.    Yiping Wu, Bintian Zhang, Liang-Hong Guo*, “Label-Free and Selective Photoelectrochemical Detection of Chemical DNA Methylation Damage Using DNA Repair Enzymes”, Analytical Chemistry, 2013, 85, 6908−6914.

6.     P.-X. Dong, B. Wan*, Z.-X. Wang, L.-H. Guo*, Y. Yang, L. Zhao, “Exposure of single-walled carbon nanotubes impairs the functions of primarily cultured murine peritoneal macrophages”, Nanotoxicology, 2013, 7, 1028-1042.

7.    Wan, B*.; Wang Z. X.; Lv, Q. Y.; Dong, P. X.; Zhao, LX; Yang, Y; Guo, L. H*., “Single-walled carbon nanotubes and graphene oxides induceautophagosome accumulation and lysosome impairment in primarilycultured murine peritoneal macrophages”, Toxicology Letters, 2013, 221, 118-127.

8.     Li, X. X.; Gao, Y.; Guo, L. H.*; Jiang, G. B., “Structure-dependent activities of hydroxylated polybrominated diphenyl ethers on human estrogen receptor”, Toxicology, 2013, 309, 15-22.

9.    Zhao, LX.*; Di, F.; Wang, DB.; Guo, L. H.*; Yang, Y.; Wan, B.; Zhang, H., “Chemiluminescence of carbon dots under strong alkaline solutions: a novel insight into carbon dot optical properties”, Nanoscale, 2013, 5, 2655-2658.

10.   Ren, X. M.; Guo, L. H.*, “Molecular toxicology of polybrominated diphenyl ethers: nuclear hormone receptor mediated pathways”, Environmental Science: Processes & Impacts, 2013, 15, 702-708.

11.  Ren, X.M.; Guo, L. H.*; Gao, Y.; Zhang, B. T.; Wan, B., “Hydroxylated polybrominated diphenyl ethers exhibit different activities on thyroid hormone receptors depending on their degree of bromination”, Toxicology and Applied Pharmacology, 2013, 268, 256-263.

12.   Y. Gao, X. Li, L.-H. Guo*, “Assessment of Estrogenic Effects of Perfluoroalkyl Acids by Monitoring Ligand-induced Conformation State of Human Estrogen Receptor”, Environmental Science & Technology, 2013, 47, 634-641.

13.   Y. Gao, L.-H. Guo*, “Development of a Label-free Competitive Ligand Binding Assay with Human Serum Albumin on Molecularly Engineered Surface Plasmon Resonance Sensor Chip”, Analytical Methods, 2012, 4, 3718-3723.

14.  M.-Y. Wei, L.-H. Guo*, P. Famouri, “Electrocatalytic oxidation of tyrosines shows signal enhancement in label-free protein biosensors”, TRAC-Trends in Analytical Chemistry, 2012, 39, 130-148.

15.   Z. Fan, Y. S. Keum, Q. X. Li, W. L. Shelver, L.-H. Guo*, “Development of indirect competitive fluorescence immunoassay for 2,2’,4,4’-tetrabromodiphenyl ether using DNA/dye conjugate as antibody multiple labels”, Journal of Environmental Science, 2012, 24, 1334-1340.

16.   B. Zhang, L.-H. Guo*, M. Greenburg*, “Quantification of 8-OxodGuo Lesions in Double-Stranded DNA Using a Photoelectrochemical DNA Sensor”, Analytical Chemistry, 2012, 84, 6048−6053.

17.   B. Zhang, L.-H. Guo*, “Highly sensitive and selective photoelectrochemical DNA sensor for the detection of Hg2+ in aqueous solutions”, Biosensor & Bioelectronics, 2012, 37, 112–115.

18.   X. M. Ren, L.-H. Guo*, “Assessment of the Binding of Hydroxylated Polybrominated Diphenyl Ethers to Thyroid Hormone Transport Proteins Using a Site-Specific Fluorescence Probe”, Environmental Science & Technology, 2012, 46, 4633−4640.

19.   P. X. Dong, B. Wan*, L.-H. Guo, “In vitro toxicity of acid-functionalized single-walled carbon nanotubes: effects on murine macrophages and gene expression profiling”, Nanotoxicology, 2012, 6, 288-303.

20.   Z. Fan, Y. S. Keum, Q. X. Li, W. L. Shelver, L.-H. Guo*, “Sensitive immunoassay detection of multiple environmental chemicals on protein microarrays using DNA/dye conjugate as a fluorescent label”, Journal of Environmental Monitoring, 2012, 14, 1345–1352.

21.   Q. Ma, G.-C. Xi, C. Wang, H. Bai, Q. Zhang, H.-W. Xi, L.-H. Guo*, “Comprehensive two-dimensional separation for the analysis of alkylphenol ethoxylates employing hydrophilic interaction chromatography coupled with ion mobility-mass spectrometry”, International Journal of Mass Spectrometry, 2012, 315, 31– 39.

22.   Y. Liu, S. Jia, L.-H. Guo, “Development of microplate-based photoelectrochemical DNA biosensor array for high throughput detection of DNA damage”, Sensors and Actuators B, 2012, 161, 334– 340.

23.   M.-Y. Wei, R. Huang, L.-H. Guo*, “High catalytic activity of indium tin oxide nanoparticle modified electrode towards electro-oxidation of ascorbic acid”, Journal of Electroanalytical Chemistry, 2012, 664, 156–160.

24.   J. Cao, L.-H. Guo*, B. Wan, Y. Wei, “In vitro fluorescence displacement investigation of thyroxine transport disruption by bisphenol A”, Journal of Environmental Sciences, 2011, 23, 315–321.

25.   Y. Yang, L.-H. Guo, N. Qu, M.-Y. Wei, L.-X. Zhao, B. Wan, “Label-free electrochemical measurement of protein tyrosine kinase activity and inhibition based on electro-catalyzed tyrosine signaling”, Biosensors and Bioelectronics,  2011, 28, 284– 290.

26.   Q. Ma, C. Wang, H. Bai, H.-W. Xi, G.-C. Xi, X.-M. Ren, Y. Yang, L.-H. Guo*, “Comprehensive Two-Dimensional Separation of Hydroxylated Polybrominated Diphenyl Ethers by Ultra-Performance Liquid Chromatography Coupled with Ion Mobility-Mass Spectrometry”, J. Am. Soc. Mass Spectrom. 2011. 

27.   Q. Zhang, S. Zhu, L.-H. Guo*, “DNA/Dye conjugates as multiple labels for antibodies in sensitive fluorescence immunoassays”, In Bioconjugation Protocols: Strategies and Methods (2nd edition), Ed. S. S. Mark, Humana Press, 2011, pp 43-54.

28.   B. Zhang, X. Du, S. Jia, J. He, L.-H. Guo*, “Rapid assessment of DNA damage induced by polystyrene nanosphere suspension using a photoelectrochemical DNA sensor”, Science China: Chemistry, 2011, 54, 1260–1265.

29.   R. Huang, M.-Y. Wei, L.-H. Guo*,“Enhanced electrogenerated chemiluminescence of Ru(bpy)32+ /tripropylamine system on indium tin oxide nanoparticle modified transparent electrode”, Journal of Electroanalytical Chemistry, 2011, 656, 136–139.

30.   M.-Y. Wei, L.-H. Guo, P. Famouri, “DNA biosensors based on metallo-intercalator probes and electrocatalytic amplification”, Microchim Acta, 2011, 172, 247–260

31.   N. Qu, L.-H. Guo, B.-Z. Zhu*, “An electrochemical biosensor for the detection of tyrosine oxidation induced by Fenton reaction”, Biosensors and Bioelectronics, 2011, 26, 2292–2296.

32.   万斌*,郭良宏, “多溴联苯醚的环境毒理研究进展”, 《环境化学》,2011, 30.

33.  Yin Wei*, Yuan Lin, Ai-Qian Zhang, Liang-Hong Guo, Jie Cao, “Evaluation of the noncovalent binding interactions between polycyclic aromatic hydrocarbon metabolites and human p53 cDNA”, Science of the Total Environment, 2010, 408, 6285–6290.

34.   J. Cao, Y. Lin, L.-H. Guo, A.-Q. Zhang, Y. Wei, Y. Yang, “Structure-based investigation on the binding interaction of hydroxylated polybrominated diphenyl ethers with thyroxine transport proteins”, Toxicology, 2010, 277, 20–28.

35.   R. Huang, L.-R. Wang, L.-H. Guo*, “Highly sensitive electrochemiluminescence displacement method for the study of DNA/small molecule binding interactions”, Anal. Chim. Acta. 2010, 676, 41–45.

36.   R. Huang, L.-H. Guo*, “Lack of nano size effect on electrochemistry of dopamine at a gold nanoparticle modified indium tin oxide electrode”, Sci. China Chem., 2010, 53, 1778–1783.

37.   S. Jia, B.-Z. Zhu, L.-H. Guo*, “Detection and mechanistic investigation of halogenated benzoquinone induced DNA damage by photoelectrochemical DNA sensor”, Anal. Bioanal. Chem. 2010, 397, 2395–2400

38.   杨郁, 郭良宏*, “超分子主体化合物环糊精应用于环境污染物分离分析的研究进展”, 《科学通报》, 2009, 54, 128 – 137.

39.   L-R. Wang, Y. Wang, J.-W. Chen, L.-H. Guo , “A structure-based investigation on the binding interaction of hydroxylated polycyclic aromatic hydrocarbons with DNA”, Toxicology, 2009, 262, 250–257.

40.   Q. Zhang, R. Huang, L.-H. Guo, “One-step and high-density protein immobilization on epoxysilane-modified silica nanoparticles”, Chinese Sci Bull, 2009, 54, 2620-2626.

41.   M.-Y. Wei, S.-D. Wen, X.-Q. Yang, L.-H. Guo*, “Development of redox-labeled electrochemical immunoassay for polycyclic aromatic hydrocarbons with controlled surface modification and catalytic voltammetric detection”, Biosensors and Bioelectronics, 2009, 24, 2909–2914.

42.   Y. Wei, L.-H. Guo*, “Binding interaction between polycyclic aromatic compounds and DNA by fluorescence displacement method”, Environmental Toxicology and Chemistry, 2009, 28, 940–945.

43.   韦明元,郭良宏*, “环境污染物的免疫传感检测方法进展’’, 《化学进展》,2009, 21, 492-502.

44.   X.-Q. Yang, L.-H. Guo*, “Enhanced Electrochemical Activity of Redox-labels in Multi-layered Protein Films on Indium Tin Oxide Nanoparticle-based Electrode”, Analytica Chimica Acta, 2009, 21, 373.

45.   Y. Chen, L.-H. Guo*, “Combined fluorescence and electrochemical investigation on the binding interaction between organic acid and human serum albumin”, Journal of Environmental Sciences. 2009, 632, 15.

46.   M. Liang, L.-H. Guo*, “Application of nanomaterials in environmental analysis and monitoring”, Journal of Nanoscience and Nanotechnology. 2009, 9, 2283.

47.   Y. Chen, L.-H. Guo*, “Fluorescence study on site-specific binding of perfluoroalkyl acids to human serum albumin”, Archives of Toxicology. 2009, 83, 255.